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研究開発について][研究開発プロジェクト][研究支援][研究成果][論文発表][学会発表
論文発表
  • ピエゾマイクロマニピュレータの特徴と応用
    土方彩子
    日本臨床エンブリオロジスト学会誌 Vol.16, No.2 1-7, 2014

  • Growh, Reproductive Performance, Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs
    Noritaka ADACHI, Daisuke YAMAGUCHI, Akiyuki WATANABE, Narumi MIURA, Seiji SUNAGA, Hitoshi OISHI, Michiko HASHIMOTO, Takatsugu OISHI, Masaki IWAMOTO, Hirofumi HANADA, Masanori KUBO and Akira ONISHI
    Rewproduction and Development Vol.60, No.2 April 2014, 100-105

  • ピエゾマイクロマニピュレータを用いた発生工学技術について
    岩元正樹
    日本生殖工学会誌 2013; Vol. 16 No. 1: 1-19.

  • Il2rg Gene-Targeted Severe Combined Immunodeficiency Pigs.
    Suzuki S, Iwamoto M, Saito Y, Fuchimoto D, Sembon S, Suzuki M, Mikawa S, Hashimoto M, Aoki Y, Najima Y, Takagi S, Suzuki N, Suzuki E, Kubo M, Mimuro J, Kashiwakura Y, Madoiwa S, Sakata Y, A.C.F. Perry, Ishikawa F and Onishi A.
    Cell Stem Cell 2012. 10, 753-758,

  • Production of cloned pigs expressing human thrombomodulin in endothelial cells.
    Yazaki S, Iwamoto M, Onishi A, Miwa Y, Hashimoto M, Oishi T, Suzuki S, Fuchimoto D, Sembon S, Furusawa T, Liu D, Nagasaka T, Kuzuya T, Ogawa H, Yamamoto K, Iwasaki K, Haneda M, Maruyama S and Kobayashi T.
    Xenotransplantation. 2012. 19:82-91.

  • Potential value of human thrombomodulin and DAF expression for coagulation control in pig-to-human xenotransplantation.
    Miwa Y, Yamamoto K, Onishi A, Iwamoto M, Yazaki S, Kobayashi T. et al.
    Xenotransplantation. 2010. 17:26-37.

  • Microinjection of serum-starved mitochondria derived from somatic cells affects parthenogenetic development of bovine and murine oocytes.
    Takeda K, Tasai M, Akagi S, Matsukawa K, Takahashi S, Iwamoto M, Srirattana K, Onishi A, Tagami T, Nirasawa K, Hanada H, Pinkert CA.
    Mitochondrion. 2010. 10:137-42. Epub 2009 Dec 11.

  • Successful cross-breeding of cloned pigs expressing endo-beta-galactosidase C and human decay accelerating factor.
    Yazaki S, Iwamoto M, Onishi A, Miwa Y, Suzuki S, Fuchimoto D, Sembon S, Furusawa T, Hashimoto M, Oishi T, Liu D, Nagasaka T, Kuzuya T, Maruyama S, Ogawa H, Kadomatsu K, Uchida K, Nakao A, Kobayashi T.
    Xenotransplantation. 2009 Nov;16(6):511-21.

  • Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation.
    Liu D, Kobayashi T, Onishi A. Furusawa T, Iwamoto M, et al.
    Xenotransplantation. 14:67-73. 2007

  • Transmission of mitochondrial DNA in pigs and progeny derived from nuclear transfer of Meishan pig fibroblast cells.
    Takeda K, Tasai M, Iwamoto M, Tagami T,Nirasawa K, Hanada H, Onishi A.
    Molecular Reproduction and Development. 73: 306-12. 2006

  • Microinjection of cytoplasm or mitochondria derived from somatic cells affects parthenogenetic development of murine oocytes.
    Takeda K, Tasai M, Iwamoto M, Onishi A, Tagami T, Nirasawa K, Hanada H.
    Biology of Reproduction. 72: 1397-404. 2005

  • Effect of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation, and development to the blastocyst stage after somatic cell nuclear transfer.
    Iwamoto M, Onishi A, Fuchimoto D, Hashimoto M, Hanada H, Nagai T, Kikuchi K.
    Zygote. 13: 335-45. 2005

  • Development to the blastocyst stage of immature pig oocytes arrested before the metaphase-II stage and fertilized in vitro.
    Somfai T, Kikuchi K, Onishi A, Iwamoto M, Fuchimoto D, Sato E, Nagai T.
    Animal Reproduction Science. 90: 307-28. 2005

  • Chromosomal instability in the cattle clones derived by somatic cell nuclear-transfer.
    Hanada H, Takeda K, Tagami T, Iwamoto M, Fuchimoto D, Onishi A, King WA.
    Molecular Reproduction and Development. 71: 36-44. 2005

  • Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage.
    Iwamoto M, Onishi A, Fuchimoto D, Takeda K, Hanada H, Nagai T, Kikuchi K, et al.
    Theriogenology. 63: 1277-89. 2005

  • A novel method for the production of transgenic cloned pigs: electroporation -mediated gene transfer to non-cultured cells and subsequent selection with puromycin.
    Watanabe S, Iwamoto M, Suzuki S, Fuchimoto D, Nagai T, Hashimoto M, Onishi A, et al.
    Biology of Reproduction. 72: 309-15, 2005

  • 体細胞クローンブタ作出効率改善の試み
    岩元正樹、菊地和弘、淵本大一郎、永井卓、橋本径子、花田博文、大西彰
    日本養豚学会誌. 41: 185-192. 2004

  • Relationship between the morphological changes of somatic compartment and the kinetics of nuclear and cytoplasmic maturation of oocytes during in vitro maturation of porcine follicular oocytes.
    Somfai T, Kikuchi K, Onishi A, Iwamoto M, Fuchimoto D, Papp AB, Sato E and Nagai T.
    Molecular Reproduction and Development. 68:484-491. 2004

  • Effect of replacement of pyruvate /lactate in culture medium with Glucose on preimplantation development of porcine embryos in vitro.
    Karja NW, Medvedev S, Onishi A, Fuchimoto D, Iwamoto M, Otoi T and Nagai T .
    The journal of Reproduction and Development. 50:587-592. 2004

  • Advanced in vitro production of pig blastocysts obtained through determining the time for glucose supplementation.
    Medvedev S, Onishi A, Fuchimoto D, Iwamoto M and Nagai T.
    The Journal of Reproduction and Development. 50: 71-76. 2004

  • Meiotic arrest maintained by cAMP during the initiation of maturation enhances meiotic potential and developmental competence and reduces polyspermy of IVM/IVF porcine oocytes.
    Somfai T, Kikuchi K, Onishi A, Iwamoto M, Fuchimoto D, Papp BA, Sato E and Nagai T.
    Zygote. 11: 199-206. 2003

  • Successful piglet production after transfer of blastocyst production by a modified in virto system.
    Kikuchi K, Onishi A, Kashiwazaki N, Iwamoto M, Noguchi J, Kaneko H, Akita T and Nagai T. Biology of Reproduction. 66: 1033-1041. 2002

  • Pig cloning by microinjection of fetal fibroblast nuclei.
    Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H and A. C. F. Perry .
    Science. 289:1188-90, 2000

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